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AutoLISP was based on the Lisp programming language. AutoLISP can be seen as a superset of LISP, allowing access to a number of language features that are not supported by pure LISP. It supports object-oriented programming and much is the same as Common Lisp.
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The KM71/E strain of the yeast Pichia pastoris was used to overproduce a recombinant human blood-clotting factor VIII (FVIII) in a 50-L fermentor. Recombinant FVIII produced in this process showed a high level of purity and was functionally active. The recombinant human FVIII was composed of two-chain A2B2 molecules, which has not been reported previously for this type of recombinant protein. The recombinant protein could be secreted to the culture medium with a yield of up to 6.0 g/L, which was the highest reported yield of a protein secreted by P. pastoris. The FVIII recombinant protein was concentrated by acetone precipitation and purified to homogeneity by a one-step anion-exchange chromatography, followed by a three-step chromatofocusing. The functional activity and the physical properties of the purified recombinant FVIII were comparable to those of FVIII isolated from plasma, indicating that the purified recombinant protein was functionally active. This work shows that recombinant FVIII produced in P. pastoris can be used as an effective treatment for hemophilia A.The Big Bang.
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